Restriction Enzymes
New England Biolabs, Inc. PstI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence C_TGCA^G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I FLTRD-25MG
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. HindIII – 10000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. BbsI – 300 units
A restriction endonuclease that recognizes the sequence GAAGACNN^NNNN_.
New England Biolabs, Inc. BmgBI – 500 units
A restriction endonuclease that recognizes the sequence CAC_^GTC.
ABclonal Technology BstEII
5...G/ G T N A C C...3 3...C C A N T G/ G...5 Isoschizomers: Eco91I BstPI EcoO65I PspEI
New England Biolabs, Inc. APE 1 – 1000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. BsgI – 50 units
A restriction endonuclease that recognizes the sequence GTGCAGNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. MspA1I – 500 units
A restriction endonuclease that recognizes the sequence CMG_^CKG.
New England Biolabs, Inc. Nb.BbvCI – 5000 units
A restriction endonuclease that recognizes the sequence CCTCA_GC.
New England Biolabs, Inc. BccI – 5000 units
A restriction endonuclease that recognizes the sequence CCATCNNNN^N_.
New England Biolabs, Inc. FspI – 2500 units
A restriction endonuclease that recognizes the sequence TGC_^GCA.